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Summary: Based on the 4th Harry Potter book/movie


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Bisulfite sequencing PCRThe primers for bisulfite sequencing PCR (BSP) were designed by EpiDesigner software (Sequenom, USA) (primers shown in Supplementary Table S1). We treated the inspected DNA (bisulfite conversion) using the EZ DNA Methylation Gold Kit (ZYMO Research, Irvine, CA, USA), per the manufacturer’s protocols. PCR was carried out using ZymoTaq PreMix (ZYMO Research) according to the manufacturer’s specifications, and the PCR product was purified using the DNA Clean Concentrator 25 Kit (Zymo Research).

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PERMISSION TO TRAIN AT AFL CLUBS: Carlton: Aaron Kite (Adel), Matt Maguire (StK). Collingwood: Daniel Frost (Collingwood, VFL), Kristopher Pendlebury (Collingwood, VFL). Fremantle: Trent Dennis Lane (Subiaco), Andrew Foster (Frem), Kyle Hardingham (East Fremantle), Matt Riggio (NM), Alex Silvagni (Casey), Danny Stanley (Coll).

Written April 22, 2012 by Alex our Customer Service Manager

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